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Ansette Digital Droplet PCR å oppdage BRAF V600E Mutasjoner i formalinfiksert parafininnstøpte Reference Standard cellelinjer
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Ansette Digital Droplet PCR å oppdage BRAF V600E Mutasjoner i formalinfiksert parafininnstøpte Reference Standard cellelinjer

Article DOI: 10.3791/53190-v • 10:16 min • October 8th, 2015
October 8th, 2015 •
1, 3, 1, 3, 4, 2, 2, 1,2
1Research Institute of Pharmaceutical Science, Department of Pharmacy, College of Pharmacy, Seoul National University, 2The Center for Anti-Cancer CDx, N-Bio, Seoul National University, 3ABION CRO, 4ABION Inc., R&D Center

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Målet med denne videoen er å vise hvordan man skal utføre automatisert DNA ekstraksjon fra formalinfiksert parafin-embedded (FFPE) referanse standard cellelinjer og digital dråpe PCR (ddPCR) analyse for å påvise sjeldne mutasjoner i en klinisk setting. Oppdager mutasjoner i FFPE- prøvene viser den kliniske nytten av ddPCR i FFPE- prøver.

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Molecular Biology DNA mutasjon BRAF ddPCR molekylær biologi fluorescens FFPE Cancer biologi
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