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Costruzione di Cell-based neurotrasmettitore fluorescenti Engineered Reporters (CNiFERs) per il rilevamento ottico dei neurotrasmettitori

Article DOI: 10.3791/53290-v • 12:48 min • May 12th, 2016
May 12th, 2016 •
1, 2, 1, 2,3, 1
1Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 2Department of Physics, University of California, San Diego, 3Section of Neurobiology, University of California, San Diego

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Vi presentiamo un protocollo per creare celle a base di neurotrasmettitore fluorescenti reporter ingegnerizzati (CNiFERs) per la rilevazione ottica del rilascio di neurotrasmettitore volumetrico.

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Neuroscienze Numero 111 imaging ottico FSCV la dialisi la trasmissione del volume i neurotrasmettitori neuropeptidi TPLSM biosensore GPCR
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