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JoVE Journal Neuroscience
Bygging av Cell-baserte Nevrotransmitter Fluorescent Engineered Reporters (CNiFERs) for optisk deteksjon av Nevrotransmittere
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Bygging av Cell-baserte Nevrotransmitter Fluorescent Engineered Reporters (CNiFERs) for optisk deteksjon av Nevrotransmittere

Article DOI: 10.3791/53290-v • 12:48 min • May 12th, 2016
May 12th, 2016 •
1, 2, 1, 2,3, 1
1Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 2Department of Physics, University of California, San Diego, 3Section of Neurobiology, University of California, San Diego

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Vi presenterer en protokoll for å skape celle-basert signalstoffet fluorescerende konstruerte journalister (CNiFERs) for optisk deteksjon av volumetrisk neurotransmitter utgivelsen.

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Neuroscience optisk imaging FSCV dialyse volum overføring nevrotransmittere nevropeptider TPLSM biosensor GPCR
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