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Chromatin Immunpræcipitation Assay til identifikation af Arabidopsis Protein-DNA interaktioner
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Chromatin Immunpræcipitation Assay til identifikation af Arabidopsis Protein-DNA interaktioner

Article DOI: 10.3791/53422-v • 12:36 min • January 14th, 2016
January 14th, 2016 •
1, 1, 1, 1
1Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid

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Kromatin immunofældning er en kraftfuld teknik til identifikation af DNA-bindingssteder fra Arabidopsis proteiner in vivo. Denne procedure omfatter chromatin tværbinding og fragmentering, immunfældning med selektive antistoffer mod proteinet af interesse og qPCR analyse af bundne DNA. Vi beskriver en enkel chip assay optimeret til Arabidopsis planter.

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Plantebiologi Developmental Biology, Chromatin immunoprecipitation (chip) transkriptionel regulering blomstringstid
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