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Kromatin Immunoprecipitation analys för identifiering av Arabidopsis Protein-DNA-interaktioner
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Kromatin Immunoprecipitation analys för identifiering av Arabidopsis Protein-DNA-interaktioner

Article DOI: 10.3791/53422-v • 12:36 min • January 14th, 2016
January 14th, 2016 •
1, 1, 1, 1
1Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid

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Kromatin immunoprecipitation är en kraftfull teknik för identifiering av DNA-bindande ställen för Arabidopsis-proteiner in vivo. Detta förfarande innefattar kromatin tvärbindning och fragmentering, immunoutfällning med selektiva antikroppar mot proteinet av intresse, och qPCR analys av bundet DNA. Vi beskriver en enkel ChIP-analys optimerad för Arabidopsis växter.

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Växtbiologi Utvecklingsbiologi, transkriptionsreglering blomningstid
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