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Echtzeit-Imaging von Plant Cell Oberflächen Dynamics mit variabler Winkel Epifluoreszenzmikroskopie
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Echtzeit-Imaging von Plant Cell Oberflächen Dynamics mit variabler Winkel Epifluoreszenzmikroskopie

Article DOI: 10.3791/53437-v • 06:31 min • December 12th, 2015
December 12th, 2015 •
1
1Graduate School of Frontier Sciences, The University of Tokyo

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Das Ziel des Protokolls ist es, zu demonstrieren, wie fluoreszenzmarkierte Proteindynamik zu Pflanzenzelloberflächen mit variablem Winkel Epifluoreszenzmikroskopie, die blinkende Punkte von GFP-markiertem PATROL1 ein Membrantransportprotein zu überwachen, in der Zelle Kortex der Stomata Komplex in Arabidopsis thaliana.

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Plant Biology Ausgabe 106, Zelloberfläche Exocytosis Fluoreszierende Proteine Bildanalyse Kymograph Membrane Handel Pflanzen Zellbiologie Echtzeit-Bildgebung Stomata Totalreflexion Mikroskopie Variable-Angle Epifluoreszenzmikroskopie
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