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JoVE Journal Bioengineering
Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
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Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Article DOI: 10.3791/53459-v • 13:16 min • December 22nd, 2015
December 22nd, 2015 •
1, 2,3,4, 2,3, 1, 4, 1,2,3,4, 1,2,3,4, 1,2,3
1Skeletal Biotech Laboratory, The Hebrew University–Hadassah Faculty of Dental Medicine, 2Department of Surgery, Cedars-Sinai Medical Center, 3Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, 4Biomedical Imaging Research Institute, Cedars-Sinai Medical Center

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Summary
Automatic Translation

Implantation of autologous and allogeneic bone grafts constitute accepted approaches to treat major craniofacial bone loss. Yet the effect of graft composition on the interplay among neovascularization, cell differentiation and bone formation is unclear. We present a multimodal imaging protocol aimed to elucidate the angiogenesis-osteogenesis interdependence at the graft proximity.

Tags

Computed Tomography Optical Imaging Osteogenesis-angiogenesis Coupling Cranial Bone Autografts Allografts Vascularization Bone-grafting Procedure Bone Autograft Blood Vessel Formation Graft Effect On Neovascularization Micro-computed Tomography (µCT) Polymerizing Contrast Agent Vascular Analysis Blood Perfusion Fluorescence Imaging (FLI) Hydroxyapatite-targeted Probe Stem Cell Recruitment Bioluminescence Imaging (BLI) Calvarial Defect Surgery Data Analysis
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