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En strategi at validere rolle Callose-medieret Plasmodesmal gating i Tropic svar
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En strategi at validere rolle Callose-medieret Plasmodesmal gating i Tropic svar

Article DOI: 10.3791/53513-v • 12:18 min • April 17th, 2016
April 17th, 2016 •
*1, *1, 1, 1, 2, 1
1Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
* These authors contributed equally

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Denne artikel beskriver metoderne til screening af gener, der kontrollerer plasmodesmal permeabilitet og dermed auxin gradient under tropiske respons. Dette omfatter måling af graden af ​​tropiske respons i hypokotyle af Arabidopsis thaliana og kontrol plasmodesmal permeabilitet med 8-hydroxypyren-1,3,6-trisulfonsyre (HPTS) lastning og vurdering endelig callose niveau.

Tags

Cellular Biology , Plasmodesmata (PD) callose auxin tropisme hypokotyle symplasmic bevægelse anilin blå 8-hydroxypyren-1,3,6-trisulfonsyre (HPTS) mutant
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