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JoVE Journal
Chemistry

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放射性标记和磷酸肌醇的细胞水平的定量高效液相色谱耦合流动闪烁
 
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放射性标记和磷酸肌醇的细胞水平的定量高效液相色谱耦合流动闪烁

Article DOI: 10.3791/53529-v 10:52 min January 6th, 2016
January 6th, 2016

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Summary

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磷酸肌醇的信号脂类的相对丰度变化迅速响应各种刺激。本文通过代谢标记的细胞 3 H- 肌醇 ,随后萃取和脱酰描述了一种用于测量磷酸肌醇的丰度的方法。萃取甘油基肌醇然后通过高效液相色谱法分离,并通过流式细胞闪烁定量。

Tags

化学,第107,磷酸肌醇,脂质,膜运输,流量闪烁,信号转导,放射性标记,液相色谱
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