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Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Article DOI: 10.3791/53555-v • 11:19 min • March 7th, 2016
March 7th, 2016 •
1, 1, 2, 2, 2, 1
1U.S. Army Center for Environmental Health Research, 2Nanohmics, Inc.

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This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial cells for use in a field portable electric cell-substrate impedance sensor. The protocol for running a rapid drinking water toxicity test with the sensor is also described.

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Biosensor Impedance-based Biosensor Drinking Water Samples Toxicity Evaluation Biochips RTgill-W1 Cells Chemical Toxicity Field Conditions Refrigeration Cell Seeding Bleach Solution Tubing Assemblies Syringe Vacuum Line
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