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A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
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A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

Article DOI: 10.3791/53557-v • 08:35 min • July 20th, 2017
July 20th, 2017 •
1, 2, 2, 2
1Department of Neurosurgery, Stanford University Hospital, Stanford University School of Medicine, 2Inova Neuroscience Institute

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Summary
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Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.

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Human Glioblastoma Organotypic Slice Culture Model Tumor Cell Migration Anti-invasive Drugs Neurooncology Patient-specific Effects Disease Progression Treatment Responses Primary Cells In Vivo Tissue Architecture Cellular Heterogeneity PTFE Culture Inserts Slice Culture Maintenance Medium Tumor Tissue Pieces Processing Medium Red Blood Cells Scalpel Necrotic Tissue
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