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JoVE Journal Neuroscience
Hele Mount Dissection en Immunofluorescentie van de Adult Mouse Cochlea
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Hele Mount Dissection en Immunofluorescentie van de Adult Mouse Cochlea

Article DOI: 10.3791/53561-v • 12:02 min • January 1st, 2016
January 1st, 2016 •
1, 1,2
1Department of Surgery, Division of Otolaryngology, Southern Illinois University, School of Medicine, 2Department of Pharmacology, Southern Illinois University, School of Medicine

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We presenteren een methode om de volwassen orgaan van Corti isoleren drie intacte cochleaire windingen (apex, midden en base). We tonen ook een procedure voor immunokleuring met fluorescent gelabelde antilichamen. Samen technieken maken het bestuderen van haarcellen, steuncellen en andere celtypen in de cochlea.

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Neurowetenschappen orgaan van Corti haarcellen het ondersteunen van cellen slakkenhuis het binnenoor temporale bot voorbereiding van het oppervlak immunohistochemie immunokleuring immunolabeling neurowetenschappen
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