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Journal / Bioengineering / Usando Tomoauto: Um Protocolo de métodos de alta…
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JoVE Journal Bioengineering
Usando Tomoauto
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Usando Tomoauto: Um Protocolo de métodos de alta capacidade automatizada Cryo-Electron Tomografia

Article DOI: 10.3791/53608-v • 11:33 min • January 30th, 2016
January 30th, 2016 •
1, 1, 1
1Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston

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Nós apresentamos um protocolo sobre como utilizar de alto rendimento tomografia crio-eletrônica para determinar alta resolução em estruturas in situ de máquinas moleculares. O protocolo permite que grandes quantidades de dados a serem processados, evita gargalos comuns e reduz o tempo de inatividade de recursos, permitindo que o usuário se concentrar em questões biológicas importantes.

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Bioengenharia Edição 107, cryotomography elétron. patógeno bacteriano minicell secreção de proteínas injectisome maquinaria molecular análise de imagem de alto rendimento
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