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JoVE Journal Developmental Biology
Isolering og dyrkning af Adult Zebrafisk Brain-afledte Neurosfærer
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Isolering og dyrkning af Adult Zebrafisk Brain-afledte Neurosfærer

Article DOI: 10.3791/53617-v • 11:01 min • February 29th, 2016
February 29th, 2016 •
*1,2, *3, 1, 1,3, 1
1Yale Cardiovascular Research Center, Internal Medicine, Yale University, 2Department of Medicine, University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière, Université Pierre and Marie Curie
* These authors contributed equally

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Her giver vi en reproducerbar metode til at undersøge voksen neurogenese ved anvendelse af en neurosfære assay afledt af hele hjernen eller fra enten telencephalic, tectal eller cerebellare regioner af den voksne zebrafisk hjerne. Desuden har vi beskrive, hvorledes det manipulere genekspression i zebrafisk neurosfærer.

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Developmental Biology zebrafisk neurogenese neurale voksne stamceller / stamceller neurosfæren assay miRNA genmanipulation
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