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Identifikation af kinesin-1 Cargos Brug Fluorescens Microscopy

Article DOI: 10.3791/53632-v • 08:06 min • February 14th, 2016
February 14th, 2016 •
1
1Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai

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Her er en protokol forelægges identificere kinesin-1 ladninger. En motorless mutant af kinesin-1 tung kæde (KIF5B) aggregater i cytoplasmaet og inducerer sammenlægning af sine laster. Begge aggregater opdages under fluorescensmikroskopi. En lignende strategi kan anvendes til at identificere laster af andre motordrevne proteiner.

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Biokemi c-myc kinesin-1 KLC1 KIF5B kinesiner myosiner menneskehandel dominant negativ fluorescerende protein fluorescens mikroskopi
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