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Journal / Biology / Identificatie van kinesine-1 Cargos Met behulp…
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Identificatie van kinesine-1 Cargos Met behulp van fluorescentiemicroscopie
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Identificatie van kinesine-1 Cargos Met behulp van fluorescentiemicroscopie

Article DOI: 10.3791/53632-v • 08:06 min • February 14th, 2016
February 14th, 2016 •
1
1Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai

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Hier wordt een protocol voorgelegd aan Kinesin-1 ladingen te identificeren. Een motorloze mutant van de kinesine-1 zware keten (KIF5B) aggregaten in het cytoplasma en induceert aggregatie van de ladingen. Beide aggregaten gedetecteerd onder fluorescentiemicroscopie. Een soortgelijke strategie kan worden toegepast om ladingen van andere auto eiwitten te identificeren.

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Biochemie c-MYC Kinesin-1 KLC1 KIF5B kinesins myosins mensenhandel dominant negatief fluorescerend eiwit fluorescentiemicroscopie
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