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Journal / Bioengineering / Melhorar 2D e 3D Pele
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JoVE Journal Bioengineering
Melhorar 2D e 3D Pele
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Melhorar 2D e 3D Pele

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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Apresenta-se um protocolo para obter matrizes derivadas de células ricas em proteínas da matriz extracelular, utilizando Crowders macromoleculares (MMC). Além disso, apresentamos um protocolo que incorpora MMC na geração de co-cultura de pele organot�ica 3D, o que reduz o tempo de cultura, mantendo a maturidade da construção.

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Bioengenharia Edição 114 queratinócitos fibroblastos apinhamento macromolecular cultura de células 2D cultura celular 3D organotípicas pele co-culturas matriz extracelular colágeno tipo I colágeno tipo IV colagénio tipo VII, dermo-epidérmica junção
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