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Developmental Biology

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JoVE Journal Developmental Biology
A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
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A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

Article DOI: 10.3791/53655-v • 08:34 min • March 3rd, 2016
March 3rd, 2016 •
1, 1, 1
1Institute of Clinical Biochemistry, Hannover Medical School

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Summary
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Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations.

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Purification Endoderm Cells Human Embryonic Stem Cells Technique Undifferentiated Cells Cell Culture Plates Basement Membrane Matrix Harvest PBS Enzyme-free Passaging Solution Reagent DMMF12 Medium Centrifugation Tube
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