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JoVE Journal Immunology and Infection
Isolamento e analisi citofluorimetrica di-Glioma infiltrazione sangue periferico mononucleari Cellule
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Isolamento e analisi citofluorimetrica di-Glioma infiltrazione sangue periferico mononucleari Cellule

Article DOI: 10.3791/53676-v • 12:52 min • November 28th, 2015
November 28th, 2015 •
1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

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Presentato ecco un metodo semplice per l'isolamento e il flusso citometria di cellule mononucleate del sangue periferico gliomi infiltranti che produce dati quantitativi dipendenti dal tempo sul numero e l'attivazione dello stato di cellule immunitarie che entrano presto microambiente tumorale al cervello.

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Immunologia Numero 105 glioma periferico cellule mononucleate del sangue (PBMC) galectina-1 (gal-1) GL26-Cit-gal1i GL26-Cit-NT Gr-1 natural killer (NK)
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