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JoVE Journal Neuroscience
High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster
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High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Article DOI: 10.3791/53716-v • 03:58 min • March 24th, 2016
March 24th, 2016 •
*1, *2, 1, 1, 1
1Department of Neurobiology, Duke University, 2Department of Pharmacology, Duke University
* These authors contributed equally

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Summary
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This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

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High Throughput Assay Egg-laying Preferences Drosophila Melanogaster Genes Neurons Choice Behavior Infectioning Eggening Choice Narrow Food Vial Fly Media Female Progenies Yeast Paste Propionic Acid Spatula Surface Of The Food Medium Wet Surface Larvae Burrowing Egg-laying Experiments Acrylic Egg-laying Chambers Plastic Sheets Bottom Surface Anesthetize Flies Carbon Dioxide Pad Recover From Carbon Dioxide Acclimate To Environment
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