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En Face Detection av nitrogenoksid og Superoxide i endothelial lag av intakte Arterier

Article DOI: 10.3791/53718-v • 08:58 min • February 25th, 2016
February 25th, 2016 •
1,2, 1,2, 2,3, 1,2, 1,2
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, 2Kidney Control of Homeostasis, Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg

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I denne artikkelen beskriver vi en tilpasset relativt enkel metode å bruke fluorescens fargestoff diaminofluorescein-2 diacetat (DAF-2DA) og dihydroethidium (DHE) for en face samtidig påvisning og visualisering av intracellulær nitrogenoksid (NO) og superoksid anion (O 2 .- ) henholdsvis i ferske isolerte intakte Aorta av en fedme musemodell.

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Molecular Biology arterier konfokalmikroskopi DHE DAF-2DA endotelet nitrogenoksid superoksydanion Vascular Biology
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