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Ex-vivo-Live-Imaging von Lungenmetastasen und deren Mikromilieu
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Ex-vivo-Live-Imaging von Lungenmetastasen und deren Mikromilieu

Article DOI: 10.3791/53741-v • 11:19 min • February 3rd, 2016
February 3rd, 2016 •
*1, *1, 1,2, 1,3
1Department of Anatomy, University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine
* These authors contributed equally

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Wir beschreiben ein relativ einfaches Verfahren zur ex vivo Echtzeit-Bildgebung der Tumorzell Stroma Wechselwirkungen innerhalb Lungenmetastasen unter Verwendung fluoreszierenden Reportern in Mäusen. Verwendung von Spinnplatten konfokale Mikroskopie, ermöglicht diese Technik Visualisierung von lebenden Zellen für mindestens 4 h und angepasst werden könnten andere entzündliche Lungenerkrankungen zu untersuchen.

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Medizin Ausgabe 108 Krebs, Datenträger konfokale Mikroskopie Spinnen Metastasen Immunzellen Mikroumgebung gentechnisch veränderten Mausmodellen.
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