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Ex vivo de imágenes en vivo de metástasis pulmonar y su microambiente
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Ex vivo de imágenes en vivo de metástasis pulmonar y su microambiente

Article DOI: 10.3791/53741-v • 11:19 min • February 3rd, 2016
February 3rd, 2016 •
*1, *1, 1,2, 1,3
1Department of Anatomy, University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine
* These authors contributed equally

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Se describe un método relativamente simple para ex vivo de imágenes en vivo de las interacciones célula-estroma tumoral dentro de la metástasis de pulmón, utilizando reporteros fluorescentes en ratones. Uso de hilado de disco microscopía confocal, esta técnica permite la visualización de células vivas durante al menos 4 horas y podría adaptarse para estudiar otras condiciones inflamatorias pulmonares.

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Medicina No. 108 Cáncer, haciendo girar el disco microscopía confocal la metástasis las células inmunes microambiente modelos de ratones genéticamente modificados.
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