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Journal / Bioengineering / Encapsulating Cytochrome c in Silica Aerogel…
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Bioengineering

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JoVE Journal Bioengineering
Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity
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Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

Article DOI: 10.3791/53802-v • 11:06 min • March 1st, 2016
March 1st, 2016 •
1, 1, 1
1Department of Chemistry & Biochemistry, Fairfield University

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This procedure describes how to encapsulate cytochrome c (cyt. c) in silica (SiO2) sol-gels, process these gels to form bioaerogels, and use these bioaerogels to rapidly recognize nitric oxide (NO) through a gas-phase reaction. This type of protocol may aid in the future development of biosensors or other bioanalytical devices.

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Encapsulating Cytochrome C Silica Aerogel Nanoarchitectures Metal Nanoparticles Gas-phase Bioactivity Sol-gels Bio Aerogels Nitric Oxide Biosensing Bio Analytical Fields Protein Encapsulation Porous Aerogel Solution Concentration Polypropylene Beaker Tetra Methoxy Silane Methanol Parafilm Magnetic Stir Bar
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