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Métodos para descobrir Uso Promotor Alternativa e regulação da transcrição de Murino Bcrp1
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Métodos para descobrir Uso Promotor Alternativa e regulação da transcrição de Murino Bcrp1

Article DOI: 10.3791/53827-v • 11:02 min • May 27th, 2016
May 27th, 2016 •
1,2, 1,3, 4, 5,6, 7, 1,3,8,9, 1,3,8,9,10,11
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Pharmaceutical Sciences, University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, 6Magee Women's Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, 8Medicine, University of Maryland School of Medicine, 9Pathology, University of Maryland School of Medicine, 10Pharmacology, University of Maryland School of Medicine, 11Experimental Therapeutics, University of Maryland School of Medicine

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Com o transportador ABC de murino Bcrp1 (ABCG2) como um exemplo, in silico protocolos são apresentados para detectar alternativos de utilização do promotor nos genes expressos em tecidos de ratinho, e para avaliar a funcionalidade dos promotores alternativos identificados utilizando ensaios de repórter.

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Medicina Edição 111 Promotor Bcrp1 uso alternativo promotor E1U testículos murino factor-1 esteroidogênica
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