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Journal / Developmental Biology / Multi-target Chromogene Whole-mount
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Developmental Biology

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JoVE Journal Developmental Biology
Multi-target Chromogene Whole-mount
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Multi-target Chromogene Whole-mount

Article DOI: 10.3791/53830-v • 10:17 min • January 31st, 2016
January 31st, 2016 •
1, 1, 1, 1
1Department of Molecular Biosciences, The Wenner-Gren Institute (MBW), Stockholm University

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We beschrijven een multi-target chromogene whole-mount in situ hybridisatie (MC-WISH) procedure in intacte Drosophila embryo's toestaan ​​gelijktijdige en specifieke detectie van drie verschillende mRNA distributie patronen door contrasterende kleur neerslagen.

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Developmental Biology Digoxigenine biotine fluoresceïne azo kleurstof Fast Blue Fast Red INT alkalische fosfatase substraat WISH fruitvlieg
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