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Produktion af Menneskelige Norovirus fremspringende domæner i
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Produktion af Menneskelige Norovirus fremspringende domæner i

Article DOI: 10.3791/53845-v • 10:46 min • April 19th, 2016
April 19th, 2016 •
1,2, 1,2, 1,2, 1,2
1Schaller Research Group, University of Heidelberg and the German Cancer Research Center, 2Department of Infectious Diseases, Virology, University of Heidelberg

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Her beskriver vi en fremgangsmåde til at udtrykke og oprense kvalitet norovirus høj udragende (P) domæner i E. coli til anvendelse i røntgenkrystallografi undersøgelser. Denne metode kan anvendes på andre calicivirus P domæner, såvel som ikke-strukturelle proteiner, dvs.., Viralt protein genom-linked (VPG), protease, og RNA-afhængige RNA-polymerase (RdRp).

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Molecular Biology Cloning norovirus P domæne proteinekspression proteinoprensning krystallisation
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