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Cancer Research

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JoVE Journal Cancer Research
Establishment of Cancer Stem Cell Cultures de Human osteossarcoma convencional
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Establishment of Cancer Stem Cell Cultures de Human osteossarcoma convencional

Article DOI: 10.3791/53884-v • 09:25 min • October 14th, 2016
October 14th, 2016 •
1, 1, 1, 2, 1, 1, 1, 3, 1, 1
1Department of Surgery and Translational Medicine (DCMT), University of Florence, 2Neurofarba Department, University of Florence, 3Department of Traumatology and General Orthopedics, Azienda Ospedaliera Universitaria Careggi

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A presença de células-tronco cancerosas (CSCs) em sarcomas ósseos foi recentemente ligada à sua patogênese. Neste artigo, apresentamos o isolamento de CSCs de culturas de células primárias obtidas a partir de biópsias humanas de osteossarcoma convencional (OS) que utilizam a capacidade dos CSCs a crescer sob condições não aderentes.

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Cancer Research Edição 116 biologia celular câncer sarcomas ósseos o osteossarcoma convencional as células-tronco do câncer o ensaio de esfera formação cultura de células 3-dimensional sarcospheres as células-tronco do câncer osteossarcoma estaminal marcadores de células as células-tronco mesenquimais células-tronco embrionárias
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