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JoVE Journal Bioengineering
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A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Article DOI: 10.3791/53885-v • 13:34 min • April 6th, 2016
April 6th, 2016 •
*1, *1, *3, 1, 1,4, 2, 3, 1,4, 1
1Department of Tissue Engineering and Regenerative Medicine (TERM), University Hospital Wuerzburg, 2Department of Cardiothoracic Surgery, University Hospital Wuerzburg, 3Department of Bioinformatics, University Wuerzburg, 4Translational Center Wuerzburg, Fraunhofer Institute Interfacial Engineering and Biotechnology IGB
* These authors contributed equally

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Summary
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We present a three-dimensional (3D) lung cancer model based on a biological collagen scaffold to study sensitivity towards non-small-cell-lung-cancer-(NSCLC)-targeted therapies. We demonstrate different read-out techniques to determine the proliferation index, apoptosis and epithelial-mesenchymal transition (EMT) status. Collected data are integrated into an in silico model for prediction of drug sensitivity.

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Combined 3D Tissue Engineered Model In Vitro In Silico Lung Tumor Model Drug Effectiveness Specific Mutational Backgrounds Non-small Cell Lung Cancer Targeted Therapies New Substances Cell Specific Responses Pre-clinical Drug Testing Physiological Relevant Environment Tumor Entities Optimize Drug Response Predictions Franziska Schmitt Research Assistant Dynamic Culture Static Culture Conditions Cell Crown Autoclaved Bioreactor Small Intestine Submucoso Plus Mucosa Scaffold RPMI Medium
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