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Journal / Developmental Biology / Metoder for å studere Mrp4 holdige makromolekylær …
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Developmental Biology

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JoVE Journal Developmental Biology
Metoder for å studere Mrp4 holdige makromolekylær Komplekser i forskrift av fibroblast Migration
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Metoder for å studere Mrp4 holdige makromolekylær Komplekser i forskrift av fibroblast Migration

Article DOI: 10.3791/53973-v • 10:43 min • May 19th, 2016
May 19th, 2016 •
*1,2, *1, 1,2
1Division of Pulmonary Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center, 2Department of Physiology, University of Tennessee Health Science Center
* These authors contributed equally

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MRP4 regulerer ulike sykliske nukleotid-avhengige signal hendelser, inkludert en nylig belyst rolle i celle migrasjon. Vi beskriver en direkte, men mangefasettert tilnærming for å løse nedstrøms molekylære mål av MRP4 resulterer i identifikasjon av en unik MRP4 interactome som spiller nøkkelroller i finjustert regulering av fibroblast migrasjon.

Tags

Developmental Biology multiresistens protein 4 (MRP4) Actin cAMP-avhengig protein kinase (PKA) Migrasjon Oppfinnsomhet Pathway Analysis (IPA) High-Content Mikros Förster resonans energi overføring (FRET).
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