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JoVE Journal Developmental Biology
Metoder för att studera Mrp4 innehållande Makromolekylär i regleringen av Fibroblast Migration
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Metoder för att studera Mrp4 innehållande Makromolekylär i regleringen av Fibroblast Migration

Article DOI: 10.3791/53973-v • 10:43 min • May 19th, 2016
May 19th, 2016 •
*1,2, *1, 1,2
1Division of Pulmonary Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center, 2Department of Physiology, University of Tennessee Health Science Center
* These authors contributed equally

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MRP4 reglerar olika cykliska nukleotid-beroende signalering händelser inklusive en nyligen klar roll i cellmigration. Vi beskriver en direkt, men olika angreppssätt för att reda ut nedströms molekylära mål av MRP4 resulterar i identifiering av en unik MRP4 interactome som spelar nyckelroller i finjusteras reglering av fibroblast migration.

Tags

Utvecklingsbiologi Multiresistens protein 4 (MRP4) aktin cAMP-beroende proteinkinas (PKA) migration Uppfinningsrikedom Pathway Analysis (IPA) High-Content Microscopy Förster resonansenergiöverföring (FRET).
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