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Journal / Bioengineering / Utarbeidelse av 3D kollagen Gels og mikro for…
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Bioengineering

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JoVE Journal Bioengineering
Utarbeidelse av 3D kollagen Gels og mikro for studier av 3D Interaksjoner
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Utarbeidelse av 3D kollagen Gels og mikro for studier av 3D Interaksjoner

Article DOI: 10.3791/53989-v • 10:24 min • May 9th, 2016
May 9th, 2016 •
1,2, 1, 1, 1, 2,3,4, 1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

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Kollagen er en kjernekomponent i ECM, og gir viktige signaler for flere cellulære prosesser som spenner fra migrasjon til differensiering og spredning. Forutsatt her er en protokoll for å bygge celler i 3D kollagen hydrogeler, og en mer avansert teknikk for generering av randomiserte eller justert kollagen matriser ved hjelp av PDMS microchannels.

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Bioteknologi Kollagen microfluidic kanaler 3D-matriser ECM kollagen justering svulstens mikromiljø
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