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JoVE Journal Bioengineering
Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates
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Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates

Article DOI: 10.3791/54004-v • 08:16 min • May 9th, 2016
May 9th, 2016 •
1, 2, 2, 2, 1
1Department of Pathology, University of Alabama at Birmingham, 2Department of Biomedical Engineering, University of Alabama at Birmingham

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Summary
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We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.

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In Vitro Three-dimensional Breast Carcinoma Surrogates Cell Cultures Histologic Sections Cell Growth Experimental Conditions Candidate Therapeutics ECM Microcentrifuge Tube PH Level Phenol Red Sodium Bicarbonate Chamber Slide
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