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Journal / Neuroscience / Whole-celle Patch-clamp Optagelser i Brain Skiver
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Whole-celle Patch-clamp Optagelser i Brain Skiver
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Whole-celle Patch-clamp Optagelser i Brain Skiver

Article DOI: 10.3791/54024-v • 07:23 min • June 15th, 2016
June 15th, 2016 •
1, 1, 1
1Department of Psychiatry, University of Texas Southwestern Medical Center

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Denne protokol beskriver de grundlæggende proceduremæssige skridt til at udføre hel-celle patch-clamp optagelser. Denne teknik tillader studiet af den elektriske opførsel af neuroner, og når udført i hjernen skiver, tillader vurdering af forskellige neuronale funktioner fra neuroner, der stadig er integreret i relativt velbevarede hjernens kredsløb.

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Neuroscience Brain skive, Hel-celle patch-clamp strøm-clamp spænding-clamp synaptisk transmission iboende ophidselse.
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