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Expression et purification de particules de virus-like pour la vaccination
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Expression et purification de particules de virus-like pour la vaccination

Article DOI: 10.3791/54041-v • 06:17 min • June 2nd, 2016
June 2nd, 2016 •
1, 1, 1
1Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia

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Ici, nous présentons un protocole pour la synthèse de particules de type viral en utilisant soit baculovirus ou systèmes d'expression de mammifères, et la purification de l'ultracentrifugation. Cette approche hautement personnalisable est utilisée pour identifier des antigènes viraux comme cibles de vaccins d'une manière sûre et flexible.

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Médecine numéro 112 Virus-like particules particule sous-virale expression mammalien expression d'insecte le baculovirus la conception de vaccins
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