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Journal / Immunology and Infection / Un passo negativo cromatografica Purificazione di
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Immunology and Infection

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JoVE Journal Immunology and Infection
Un passo negativo cromatografica Purificazione di
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Un passo negativo cromatografica Purificazione di

Article DOI: 10.3791/54043-v • 10:44 min • June 18th, 2016
June 18th, 2016 •
1, 1, 1, 1, 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

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Un metodo ad alto rendimento per un solo passaggio di purificazione negativo ricombinante Helicobacter pylori neutrofili-attivazione di proteine ​​(HP-NAP) sovraespresso in Escherichia coli utilizzando resine dietilamminoetil in modalità batch è descritta. HP-NAP purificata da questo metodo è utile per lo sviluppo di vaccini, farmaci, o diagnostica per H. pylori -associated malattie.

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Immunologia HP-NAP, DEAE cromatografia Negativo frazione libera Batch cromatografia,
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