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Immunology and Infection

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JoVE Journal Immunology and Infection
De un solo paso negativo Purificación cromatográfica de
Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
Journal (Immunology and Infection)
Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
Journal (Immunology and Infection)
Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
 
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De un solo paso negativo Purificación cromatográfica de

Article DOI: 10.3791/54043-v • 10:44 min • June 18th, 2016
June 18th, 2016 •
1, 1, 1, 1, 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

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Un método de alto rendimiento para la purificación negativa de un solo paso de Helicobacter pylori recombinante proteína activadora de neutrófilos (HP-PAN) sobreexpresado en Escherichia coli mediante el uso de resinas de dietilaminoetilo en modo batch se describe. HP-PAN purificado por este método es beneficioso para el desarrollo de vacunas, medicamentos o diagnósticos para H. pylori -asociado enfermedades.

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Inmunología No. 112 HP-PAN, DEAE cromatografía negativo fracción no unida cromatografía por lotes,
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