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Microiniezione per Transgenesi e Genome Editing in Threespine spinarelli
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Microiniezione per Transgenesi e Genome Editing in Threespine spinarelli

Article DOI: 10.3791/54055-v • 08:51 min • May 13th, 2016
May 13th, 2016 •
1, 1, 1
1Department of Molecular and Cell Biology, University of California, Berkeley

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Manipolazioni transgeniche e la modifica del genoma sono fondamentali per il collaudo funzionale i ruoli dei geni ed elementi -regulatory cis. Ecco una dettagliata protocollo microiniezione per la generazione di modifiche genomiche (compresi costrutti Tol2-mediate fluorescenti giornalista transgene, Talens, e CRISPRs) è presentato per il pesce modello emergente, il Spinarello.

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Biologia dello Sviluppo Numero 111 spinarello microiniezione TALEN CRISPR la modifica del genoma Tol2 enhancer,
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