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Journal / Medicine / C2C12 Myoblast कक्ष में मायोटोनिक अपविकास 1…
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C2C12 Myoblast कक्ष में मायोटोनिक अपविकास 1 मॉडलिंग
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C2C12 Myoblast कक्ष में मायोटोनिक अपविकास 1 मॉडलिंग

Article DOI: 10.3791/54078-v • 09:39 min • July 29th, 2016
July 29th, 2016 •
*1, *1,3, 1, 1,3, 1,2, 1
1Department of Biology and Biochemistry, University of Houston, 2Department of Economics, University of Houston, 3Department of Cardiology, The First Affiliated Hospital of Nanchang University
* These authors contributed equally

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इस प्रोटोकॉल में, हम अनुकूलित C2C12 सेल रखरखाव, जीन अभिकर्मक / पारगमन, और myocyte भेदभाव सहित मायोटोनिक कुपोषण 1 myoblast मॉडल, स्थापित करने में प्रक्रियाओं प्रस्तुत करते हैं।

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चिकित्सा अंक 113 मायोटोनिक डिस्ट्रोफी C2C12 myoblast Celf1 प्लाज्मिड अभिकर्मक रेट्रोवायरल पारगमन lentiviral पारगमन immunostaining
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