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Modellazione Distrofia miotonica 1 nelle cellule dei mioblasti C2C12
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Modellazione Distrofia miotonica 1 nelle cellule dei mioblasti C2C12

Article DOI: 10.3791/54078-v • 09:39 min • July 29th, 2016
July 29th, 2016 •
*1, *1,3, 1, 1,3, 1,2, 1
1Department of Biology and Biochemistry, University of Houston, 2Department of Economics, University of Houston, 3Department of Cardiology, The First Affiliated Hospital of Nanchang University
* These authors contributed equally

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In questo protocollo, vi presentiamo le procedure nella creazione di modelli miotonici mioblasti distrofia 1, tra cui ottimizzato manutenzione C2C12 cellule, geni trasfezione / trasduzione, e la differenziazione dei miociti.

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Medicina la distrofia miotonica mioblasti C2C12 Celf1 plasmide trasfezione trasduzione retrovirale trasduzione lentivirale immunostaining
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