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Detecção de Anastasis na Vivo por CaspaseTracker Biosensor
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Detecção de Anastasis na Vivo por CaspaseTracker Biosensor

Article DOI: 10.3791/54107-v • 20:16 min • February 1st, 2018
February 1st, 2018 •
1,2, 2, 3
1Institute for Basic Biomedical Sciences, Johns Hopkins University School of Medicine, 2School of Life Sciences, Chinese University of Hong Kong, 3Department of Neurosurgery, Johns Hopkins University School of Medicine

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Anastasis é tecnicamente desafiador para detectar na vivo porque as células que reverteram o processo de morte celular podem ser morfologicamente indistinguíveis de células saudáveis normais. Aqui descrevemos os protocolos para detecção e monitoramento de células que passam por anastasis em animais vivos, usando nosso sistema de biosensor recentemente desenvolvido na vivo CaspaseTracker.

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Medicina edição 132 Anastasis apoptose autofagia biosensor caspase CaspaseTracker necrose necroptosis programou a morte celular reversão da apoptose
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