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Journal / Developmental Biology / Isolering av Murine Embryonic Hemogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering av Murine Embryonic Hemogenic endotelceller
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Isolering av Murine Embryonic Hemogenic endotelceller

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Blodkreft stamceller og stamceller (HSPC) stammer fra spesialiserte (hemogenic) endotelceller under utvikling, men lite er kjent om prosessen der noen endotelceller spesifisere å bli bloddannende. Vi viser en flow-cytometri basert fremgangsmåte som tillater samtidig isolering av hemogenic endotelceller og HSPC fra muse-embryonale vev.

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Developmental Biology hemogenic endotelet definitive hematopoiesis vaskulær utvikling museembryoer FACS blodkreft stamceller og stamceller
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