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Journal / Developmental Biology / Isolering av murina embryonala Hemogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering av murina embryonala Hemogenic endotelceller
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Isolering av murina embryonala Hemogenic endotelceller

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Hematopoietiska stam- och progenitorceller (HSPC) härrör från specialiserade (hemogenic) endotelceller under utveckling, men lite är känt om den process genom vilken en del endotelceller anger att bli blodbildande. Vi visar en flödescytometri baserad metod tillåter samtidig isolering av hemogenic endotelceller och HSPC från murina embryonala vävnader.

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Utvecklingsbiologi hemogenic endotel definitiva hematopoes vaskulär utveckling musembryon FACS hematopoetiska stam- och progenitorceller
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