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Journal / Biology / La détection des protéines liant l'ARN par
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La détection des protéines liant l'ARN par

Article DOI: 10.3791/54207-v • 10:34 min • July 22nd, 2016
July 22nd, 2016 •
*1, *1, 3, 1,2
1Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 2Institute of Molecular and Cell Biology, Singapore, 3Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University
* These authors contributed equally

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Un protocole de pull-down ARN est optimisée ici pour la détection d'interactions entre les protéines liant l'ARN (RBP) et non codantes ainsi que ARNs codant. Un fragment d'ARN du récepteur des androgènes (AR) a été utilisé comme un exemple pour montrer comment récupérer son RBP de lysat des adipocytes bruns primaires.

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Molecular Biology numéro 113 ARN pull-down l'ARN-protéine de liaison adipocyte non codante codant
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