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Journal / Biology / Detektering av RNA-bindande proteiner genom
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Detektering av RNA-bindande proteiner genom
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Detektering av RNA-bindande proteiner genom

Article DOI: 10.3791/54207-v • 10:34 min • July 22nd, 2016
July 22nd, 2016 •
*1, *1, 3, 1,2
1Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 2Institute of Molecular and Cell Biology, Singapore, 3Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University
* These authors contributed equally

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En RNA neddragnings protokoll är optimerad här för detektion av växelverkan mellan RNA-bindande proteiner (RBP-anordningama) och icke-kodande samt kodande RNA. En RNA-fragment från androgenreceptorn (AR) användes som ett exempel för att visa hur man hämtar sin RBP från lystate av primära bruna adipocyter.

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Molecular Biology RNA pull-down RNA-bindande protein fettceller icke-kodande kodning
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