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Journal / Developmental Biology / Oppdragelse bananflue
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Developmental Biology

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JoVE Journal Developmental Biology
Oppdragelse bananflue
A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
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A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
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Oppdragelse bananflue

Article DOI: 10.3791/54219-v • 09:34 min • July 30th, 2016
July 30th, 2016 •
1, 1, 1, 2,4, 2,5, 2,3, 1,2
1Department of Plant and Wildlife Sciences, Brigham Young University, 2Department of Entomology, Cornell University, 3Department of Molecular Biology and Genetics, Cornell University, 4Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, 5Biological Sciences, SUNY Oswego

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En fremgangsmåte for oppdrett Drosophila melanogaster henhold axenic og gnotobiotiske betingelser er presentert. Fly embryoer er dechorionated i natriumhypokloritt, overføres aseptisk til sterilt kosthold, og alet opp i lukkede beholdere. Inoculating kosthold og embryoer med bakterier fører til gnotobiotiske foreninger, og bakteriell tilstedeværelse er bekreftet av plating hele kroppen Drosophila homogenates.

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Developmental Biology axenic gnotobiotiske, Mikrobiomer microbiota,
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