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JoVE Journal Developmental Biology
Isolering av perivaskulär multi Precursor cellpopulationer från Human hjärtvävnad
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Isolering av perivaskulär multi Precursor cellpopulationer från Human hjärtvävnad

Article DOI: 10.3791/54252-v • 08:15 min • October 8th, 2016
October 8th, 2016 •
1, 2,3, 4, 4, 4, 5,6, 4,7
1Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery, University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering, Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine, University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, 6Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine, University of California at Los Angeles

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Human hjärtvävnad hyser multi perivaskulära prekursor cellpopulationer som kan vara lämpliga för hjärtmuskelregenerering. Den teknik som beskrivs här gör det möjligt för den samtidiga isolering och rening av två multipotenta stromala cellpopulationer som är förknippade med nativa blodkärl, dvs. CD146 + CD34 - pericyter och CD34 + CD146 - adventitiala celler, från den mänskliga hjärtmuskeln.

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Utvecklingsbiologi Pericyte adventitial cell blodkärl stamcells stamceller hjärtföregångarcell hjärtmuskeln hjärt regeneration flödescytometri
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