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JoVE Journal
Neuroscience

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荧光活化细胞分选(FACS)和新鲜和冷冻大鼠脑组织的Fos表达神经元基因表达分析
 
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荧光活化细胞分选(FACS)和新鲜和冷冻大鼠脑组织的Fos表达神经元基因表达分析

Article DOI: 10.3791/54358-v 08:37 min August 27th, 2016
August 27th, 2016

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Summary

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Click here for the English version.

在这里,我们提出了一个荧光活化细胞分选(FACS)协议来研究分子改建的Fos表达来自新鲜和冷冻的脑组织神经元歌舞团。使用冷冻组织可在多个会话许多脑区的FACS隔离,最大限度地利用宝贵的动物主题。

Tags

神经科学,第114类型的细胞,神经元,神经胶质细胞,抗体,基因,流式细胞仪
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