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JoVE Journal Neuroscience
Fluorescence Activated Cell Sorting (FACS) und Genexpressionsanalyse von Fos-exprimierenden Neurone von Frische und Gefrorene Rattenhirngewebe
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Fluorescence Activated Cell Sorting (FACS) und Genexpressionsanalyse von Fos-exprimierenden Neurone von Frische und Gefrorene Rattenhirngewebe

Article DOI: 10.3791/54358-v • 08:37 min • August 27th, 2016
August 27th, 2016 •
*1, *1, 1, 2, 1
1Behavioral Neuroscience Research Branch, Intramural Research Program, NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine, Johns Hopkins University
* These authors contributed equally

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Hier präsentieren wir eine Fluorescence Activated Cell Sorting (FACS) Protokoll zu untersuchen molekulare Veränderungen in Fos-exprimierenden neuronalen Ensembles aus frischen und gefrorenen Hirngewebe. Die Verwendung von gefrorenem Gewebe ermöglicht FACS Isolierung von vielen Bereichen des Gehirns über mehrere Sitzungen hinweg die Verwendung von wertvollen tierischen Patienten zu maximieren.

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Neuroscience Ausgabe 114 Zelltypen Neuronen Gliazellen Antikörper mRNA FACS
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