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Journal / Environment / Utvinning og analyse av Microbial fosfolipid…
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JoVE Journal Environment
Utvinning og analyse av Microbial fosfolipid fettsyrer i jordsmonn
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Utvinning og analyse av Microbial fosfolipid fettsyrer i jordsmonn

Article DOI: 10.3791/54360-v • 10:03 min • August 26th, 2016
August 26th, 2016 •
1, 2, 1, 3, 4, 5
1Department of Renewable Resources, University of Alberta, 2Department of Science, Augustana Faculty, University of Alberta, 3Laboratoire Génie Civil et géo-Environnement, Université de Lille, 4Department of Earth and Environmental Sciences, Mount Royal University, 5Forest Ecology & Production, Great Lakes Forestry Centre, Natural Resources Canada

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Fosfolipid fettsyrer gir informasjon om strukturen av jord mikrobielle samfunn. Vi presenterer fremgangsmåter for utvinning av jordprøver med en enfaset kloroform blanding, fraksjonering av ekstraherte lipider ved hjelp av fastfase-ekstraksjon kolonner, og metanolyse for å fremstille fettsyremetylestere, som analyseres ved kapillar gasskromatografi.

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Environmental Sciences mikrobiell samfunnsstruktur fosfolipid fettsyrer PLFA jord mikrober jord biologi mikrobiell økologi arealbruk arealforvaltningen
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